C. Thuilliez, M. Perron-Lepage, C. Clément, C. Botteron
The standard procedure for microscopic evaluation of intramuscular injection sites at our Laboratory involves the examination of three tissue pieces from each site, in order to maximise the chance of examining the area where the injected material was deposited.
Elucidation of Cytokine Storm: The Effect of In-Vitro Antibody Immobilization Methods on
Cytokine Secretion in a Cell-Based Assay
J. Mulligan, R. Shively, and C. O’Day
Cytokine storm is a severe, potentially life-threatening immune reaction resulting from an abnormal cascade of pro-inflammatory cytokine release. In a typical inflammatory event, cytokines released in response to a biological threat are kept in check by a feedback loop.
Characterization of Kinase Inhibitors by In Vitro and Functional Cellular Assays[link to (open in new window)]
G. Castillo, J. Hnilo, S. Bounds, M. Gross, K. Yoshino and L. Martel
Abnormal protein phosphorylation has been implicated in the etiology or progression of a broad spectrum of major disorders and diseases. The drug discovery industry has embraced kinase screening with the goal of finding inhibitors of clinically relevant kinases.
Discovery P450 Inhibition Screening: How Does A 5-in-1 LC-MS/MS Assay Compare to Commonly Used Fluorometric Assays?
K. Rogers, J. Woodson and D. Mautz
In this study, we compare five commonly used fluorometric assays that use expression system microsomes to an LC-MS-based method that utilizes pooled human liver microsomes and a 5-in-1 substrate cassette. The apparent advantages of the latter method are its similarity to single substrate methods used in preclinical evaluation and the uniformity of the assay conditions.
An Efficient Scalable Process for the Synthesis of (+)-trans-4-1(1-Benzyloxycarbonylaminoethyl)-cyclohexanecarboxylic Acid
D. Gopal, K. Maslanka, S. Krumins, H. Wu, B.Wicks, J. McRea, W. Weiner
The process development of a route for preparing(+)-trans-4-1(1-benzyloxycarbonylaminoethyl)-cyclohexanecarboxylic acid in kilogram quantities is described 25% overall yield using ®-(+)-1-phenyl-ethylamine as the starting material.
Evaluation of Cardiac Liability of Drugs by Two In Vitro Functional Assays
S. Wang, T. Lin, K. Bernards, Y.Ovechkina, C. O’Day and D. Small
Drug induced cardiotoxic effects including delayed cardiac repolarization may induce arrhythmias such as Torsades de Pointes or even sudden death. It is a challenge to detect these side effects of compounds at the early stage of drug development in the pharmaceutical industry.
Multiparametric Hepatotoxicity Screening in HepG2 Cells with Comparison in Primary Hepatocytes
K. Marcoe, Y. Ovechkina, R. Keyser, P. Nguyen, C. O’Day
Recognition of drug-induced hepatotoxic potential early in the drug development cascade creates opportunities for ranking and prioritizing, or developing alternatives with lower toxicity. Detection of hepatic apoptosis induction, phospholipidosis and neutral lipid accumulation late in the drug development pipeline has contributed to late-stage drug failures.