Microbiology

Mode of Action Studies

• Thymidine, uracil, methionine and DAP incorporation studies
• Synergy studies
• Over and under-expression of target gene

Whole Cell Assays

• MIC determination ­ NCCLS methods
  - Microtiter
  - Agar dilution
  
• In vitro Spectrum
  - Bacteria - Gram-positives, Gram-negatives
  - Yeasts
  - Fungi
  
  ——Fresh clinical isolates as well as ATCC standards
  
  
• Minimum Bactericidal/Fungicidal Concentration (MB/FC)

Frequency of Resistance Development

• Systemic infection - Mouse protection test
  
• Preliminary LD₅₀ and Pharmacokinetics (subcutaneous and oral administration)
  
• Additional models
  ­ Thigh infection
  ­ Pneumonia model
  ­ Clostridium difficile-associated diarrhea