Molecular Biology

• Genomic and Plasmid DNA Preparations



• Vector Constructions



• Target Gene Cloning and Expression (bacterial and mammalian):
  ­ - Clone the gene of interest in appropriate vectors
  ­———for constitutive or inducible expression
  ­ - Validate the DNA sequence
  ­ - Validate gene expression - transcription, translation
  ­ - Optimize for high/low expression
  ­———strain selection, promoter & copy number modification
• Target Gene Validation
  ­ - Check essentiality of the gene function for bacterial viability by gene knockouts
• Mutant Strain Constructions
• Transcription and Translational Analysis
• PCR
• Protein Isolation
• Immunoprecipitation
• Western blots